P-143 Will Use of Testicular sperm in ICSI cycles increase Embryo Aneuploidy?

Abstract Study question Will use of surgically retrieved Testicular Sperms (TESA) in Intra-Cytoplasmic Sperm Injection (ICSI) cycles increase embryo aneuploidy? Summary answer Use TESA sperms for ICSI seems safe and doesn’t aneuploidy. performed various indications also showed no variation with What is known already Invention improved surgical sperm retrieval techniques have helped men severe male factor infertility to father their own genetic child. from seminiferous tubules testes are considered immature as they haven’t completed the process spermiogensis. It hypothesized that can aneuploidy, which still uncertain. design, size, duration This retrospective study conducted at a private fertility clinic 2014–2022 (n = 265 patients; total 860 embryos evaluated). populationTESA used PGT-A done 66). group was further sub-divided into 2 groups based on indication TESA,Non-Obstructive Azoospermia(n 26) Raised SDF(n 40). Control Population Ejaculated 199) Only younger women(<37yrs) self-gametes RIFconsidered this study. RIF defined women failed IVFcycles thepast least 4 blastocystsbeing transferred. Participants/materials, setting, methods All fertilized oocytes inseminated by subjected extended blastocyst culture. per our clinic’s SOP control preparation density gradient method. Trophectoderm biopsy sent lab. Next-Generation sequencing (NGS) Comprehensive Chromosomal technique (CCS) assessing ploidy status. Aneuploidy across all compared. Main results role chance Following were rates sperm, Ejaculate NOA SDF respectively. – Aneuploidy%- 42% (irrespective indication)- 44% (p 0.6260) (done NOA)- 0.7504) raised SDF) 32% 0.05) Embryo aneuploidy comparable between group. Though immature, it didn’t seem or altering trend. Data suggestive alter Limitations, reasons caution small sample size unequal distribution. A well designed Randomized Trial might help test inference further. Wider implications findings Incidence rise globally. Interventions optimize implantation reproductive outcomes will better success shorten conception. Further research warranted improve selection outcomes. registration number not applicable